Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPAILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS-KGSHLTKTVIIAMGGGAFKPRPLELEGVEDYE--NIH-YHVSNIQQYAGKKVTILGGGDSAVDWALAFEKIAP-TTLVHRRDNFRAL-EHSVQALQESSVTIKTPFAPSQLLGDGKTLDKLEITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLN-RHKIIVNSKQESSQAGIYAIGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL 3F8P Chain:D ((13-319)) -GEKFDVIIVGLGPAAYGAALYSARYMLKTLVIGET--PGGQLTEAGIV---DDYLGLIEIQASDMIKVFNKHIEKYEVPVLLD-IVEKIENRGDEFVVKTKRKGEFKADSVILGIGVK---RRKLGVPGEQEFAGRGISYCSVCDAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGD-KVVKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYVTEK-----------

General information: TITO was launched using: RESULT: Template: 3F8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1798 -43758 -24.34 -145.86 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -24.34 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3F8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8P-query.scw PDB file : Tito_Scwrl_3F8P.pdb: