Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQGFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEASFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEKKD 1GN8 Chain:B ((2-150)) --QKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKPMFTLEERVALAQQATAHLGNVEVVGFSD-LMANFARNQHATVLIRGLRAVADFEYEMQLAHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENV----------

General information: TITO was launched using: RESULT: Template: 1GN8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 701 -99062 -141.31 -664.84 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -141.31 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_1GN8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GN8-query.scw PDB file : Tito_Scwrl_1GN8.pdb: