Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATPFWYNTALHLLKPFYRWRIKRRAESLELYQQECLERFGPFEAPKNVKAIWFHAVSVGETNAAQPLIEYYLKL-GQPVLVTNTTKTGQARAKSLFLKEPYLDLFQAV-YLP------------VDQKPLLKKFFELYQPKLLALVETELWPN-LIDQAKLQHVPCLLLNARLSEKSAKGYGKVSGLTAGMLKQLDWVLAQDSATRQRYVELGLDEHKSQVVGNIKFDIHAPEAFIKQAAQLRQQWYLE--NRQVVTIASTHAP-EEQQILEAFAPYLNSDRELVCIVVPRHPER-FDEVFEICQNLNLITHRRSMGQSIHASTQVYLADS--MGELWLWYALSQVCFVGGSLNEPGGGHNILEPMVLNVPTVVGPRYFNFQTIVDEFIDENAVLIAQDAQQVVDIWLACLAEPEATEQLVAQAHKVLQRNQGSLQKHIGVINRYLAEKS 1V4V Chain:A ((8-367)) ---------------------------------------------------VVLAFGTRPEATKMAPVYLALRGIPGLKPLVLLTGQHREQLRQ-ALS-L---FGIQEDRNLDVMQERQALPDLAARILPQAARALKEMGADYVLVHGDTLTTFAVAWAAFLEGIPVGHVEAGLRSGNLKEPFPEEANRRLTDVLTDLDFAPTPLAKANLLKEGKREEGILVTGQTGVDAVLLAAKL-----GRLPEGLPEGPYVTVTMHRRENWPLLSDLAQALKRVAEAFPHLTFVYPVHLNPVVREAVFPVLK----------------GVRNFVLLDPLEYGSMAALMRASLLLVTDS-----G--GLQEEGAALGVPVVVLRNVTERPEGLKA--GILKLAG-TDPEGVYRVVKGLLENPEELSRMRKAKNPYGD--GKAGLMVARGVAWRLGL--

General information: TITO was launched using: RESULT: Template: 1V4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1783 -4342 -2.44 -12.81 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -2.44 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_1V4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V4V-query.scw PDB file : Tito_Scwrl_1V4V.pdb: