Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLNFRMIPKDQYPSDVKILRVVSINVNGLRS--SVTKGLLEWLEQSDADVVCMQESRITHEQWTEKFRPEGWHTHLFPAERAGYAGTAIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVHIASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSIIICGDYNIVHKRIDIKNWSGNQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTE-AELYSWWSNRGQARAKNVGWRIDYHACSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE 5CFE Chain:A ((1-252)) -------------------MKLISWNVNGLRAVMRKM-DFLSYLKEEDADIICLQETKIQDGQVDL--QPEDYHVYWNYAVKKGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEF-----ENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILEL-DQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRF-LEAGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMGSDHCPVELIINI--

General information: TITO was launched using: RESULT: Template: 5CFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1389 656 0.47 2.63 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 0.47 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_5CFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CFE-query.scw PDB file : Tito_Scwrl_5CFE.pdb: