Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIKLNPLNAIDFYKADHRRQYPAGTEYVYANFTPRSSRLAKMLPDFDDKVVFFGLQGFIKHFLIDTWNEGFFKQPKDKVVAAYKRRM-DSSLGEGAVPVDHIEALHDLGYLPLRIKALPEGSRVNMRVPVLTVINTDPRFFWLTNYIETVLSAELWKSCTTATIAYEYKRLLTQYAVKTGAPLDFVPVQGHDFSSRGMSGIYDAAQSGVGHLTSFIGTDSVASIDYAEEYYNATG-VIGVSVPATEHSVMCMGTEDNELETFKRLICELYPSGVVSIVSDTWDFWRVITE-FTVALKPEILARQPNALGLAKLVFRPDSGDPVKIICGDPDAKVGSPAYKGAVECLWEVFGGTTTDQGYKVLNECVGLIYGDSITLDRAQRILEGLETKGFASNNLVFGIGSFTYNYLTRDTFGFAVKATWGQVNGVGRELFKDPITDSGVKKSAKGLLRIEESENG-FTLFDQQTAEQEQGGA--LKTVFENGKLQYECTLDQIRERLSIA 2E5C Chain:A ((16-490)) ---EFNILLATDSYKVTHYKQYPPNTSKVYSYFECREK--------KYEETVFYGLQYILNKYLK---GKVVTKEKIQEAKDVYKEHFQDDVFNEKGW--NYILEKYD-GHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETIL-VQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQ-FSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQA----PLIIRPDSGNPLDTVLK-------------VLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPN-KRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQL-

General information: TITO was launched using: RESULT: Template: 2E5C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2509 29699 11.84 64.70 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 11.84 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_2E5C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E5C-query.scw PDB file : Tito_Scwrl_2E5C.pdb: