Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVAMSIMISTEEIQAKVKELGEQINSHYANSDKELVLIGLLRGSVIFMADLCRTITKPHELDFMTVSSYGGGTTSSRDVKILKDLDGEIRGKDVLVVEDIIDSGNTLSKVVEMLQTREPNSIQLCTLVCKPSRREIDLEVKFLGFEVEDKFIVGYGLDYDQKYRHLPFIGEIGL 4RHT Chain:D ((24-195)) -----SVLLTAEQIQARIAELGEQIGNDY----QDLLLITVLKGAVLFVTDLARAIPVPTQFEFMAVSSYGS----SGVVRILKDLDRDIHGRDVLIVEDVVDSGLTLSWLSRNLTSRNPRSLRVCTLLRKPD---ANVEIAYVGFDIPNDFVVGYGLDYDERYRDLSYIGTL--

General information: TITO was launched using: RESULT: Template: 4RHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 746 -132180 -177.18 -841.91 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.88 3D Compatibility (PKB) : -177.18 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_4RHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RHT-query.scw PDB file : Tito_Scwrl_4RHT.pdb: