Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVIDFKQDILAPVATDFAAMDHLINEGISSKVGLVMSVSKHVVEAGGKRMRPIMCLLAARACGLDNMQNAQRLAAIIEMLHTATLVHDDV--VDESGLRRGRPTANATWNNQTAVLVGDFLIARAFDLLVDLNNMT-----LLKDFS--TGTCEIAEGEVLQLQSQHQPDTTEETYLKIIH-GKTSRLFELATEGAAILAGQEAYREPLRLFAGH-FGNAFQIIDDILDYTSDAETLGKNIGDDLMEGKPTLPLISALAHSTGEEHAIIRRSIATGGVDQLPKVIEIVQKSGALDYCQRRAQEETEAALQALSILPDTPYRQALINLTRLALHRIQ 4LFG Chain:A ((32-250)) ----------------------------------------YSINAGGKRIRPILFLEVIEALQIPLTESHFKAAAALEMIHTGSLIHDDLPAMDNDDYRRGQLTNHKKFDEATAILAGDSLFLDAFGMLAETDFPTDVTVDLVRSLSSASGTFGMVGGQMLDMAAEGKKLNLKN--LQLIHRHKTGQLLAYPFWAAARVAQLDENLLATFLEIGMIIGLAFQVRDDILDITANFEEIGKTPKKDVMAEKMTYPHLLGLNES---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 899 -5965 -6.64 -28.68 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -6.64 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_4LFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LFG-query.scw PDB file : Tito_Scwrl_4LFG.pdb: