Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKGASPMKAVTWQGKQKMEI-RNVDDPKILEPTDAIIRITATAICGSDLHLYHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMESQCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRV-PYADSTSFVVPDNDLPDEKVLFLSDILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKAANYNGVET-YFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGTISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYAMIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP 4C4O Chain:B ((2-336)) ---SIPSSQYGFVFNKQSGLNLRNDLPVH-KPKAGQLLLKVDAVGLCHSDLHVIYEGLD-CGDNYVMGHEIAGTVAAVGDDVINYKVGDRVACVGPNGCGGCKYCRGAIDNVCKNAFG-----DWFGL-----GYDGGYQQYLLVTR--PRNLSRIPDN-VSADVAAASTDAVLTPYHAIKMAQVSPTSNILLIGAGGLGGNAIQVAKAFGA-KVTVLDKKKEARDQAKKL-GADAVYETLPE--------SISPGSFSACFDFVSVQ--------------------ATFDVCQKYVEPKGVIMPVGLGAPN-LSFNLGDLALREIRILGSFWGTTNDLDDVLKLVSEGKVKP--VV-RSAKLKELPEYIEKLRNNA-YEGRVVFNP

General information: TITO was launched using: RESULT: Template: 4C4O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2057 -55377 -26.92 -166.80 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -26.92 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_4C4O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C4O-query.scw PDB file : Tito_Scwrl_4C4O.pdb: