Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMTMSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYYKQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIFHGGRMTSRATLSGEQPVSASAVAAKR-------PDAETPRAMSEEEIVETIQAFGKATRRAIESGFDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKPFIVGYRFSPEEYETPGIRFED---TIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSILEVVHNTIQGKIPLVGVGGIRNREDVEQVLK-Y-AELAAIGQQMIVDPDWAEKLLENREDEFVTKPFEEAYKELYLPSPLYNFLKARYQ 4B5N Chain:A ((7-344)) --VNSVENLFDTYKLNDTITLKNRILMAPLTRCMADANLVPTDDMVAYYARRAE-AGLIISEATIIRPDAQGYPNTPGIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAPSAQKIEGSVPRMRELTYVTPKAVTVEDIQGLVRDYAKAAENVIEAGFDGVEIHGANGYLIDQFLHHDSNRRTDEYGGTPVNMSRFALEVVDAIIARIG-----HDRTGLRISPGAYFNMASDSRDRVVFDYLLPELEKRDLAFVHIGIFDDSIE-FDY--LGGTASSYVRAHY--GKTLVGVGSY-SAETASKAIAEDKFDLIAIGRPFIANPDYVAKVRNSEELV----------------------------

General information: TITO was launched using: RESULT: Template: 4B5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1998 -22651 -11.34 -69.48 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -11.34 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_4B5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B5N-query.scw PDB file : Tito_Scwrl_4B5N.pdb: