Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWIAHKMDMSMKLIHQAERYLAEKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDVYLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLVKEMRKRQEERRVNDENSK 2W0C Chain:T ((1-127)) ------MANFLTKNFVWILAAGVGVWFYQKADNAAKTATKPIADFLAELQFLVNGSN-YVKFPNAGFVLTRDALQDDFIAYDDRIKAWLGTHDRHKDFLAEIL---------------------DHERRVKPVYRKLIGNIIDASTIRAASGVEL-

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 131 3730 28.47 29.37 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain T : 0.57 3D Compatibility (PKB) : 28.47 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: