Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTVHVDNQKYDIHIEKGALEQ-AGS-WLQTIWRPQKVAVITDTNVAPLYAEKIMFQLRQANF------EPVLSIVPAGETSKSLEQAAELYDFLADND--FTRSDGILALGGGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPAGVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKD----------------LLEHAVPIITATLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHAEKIGLSPTGSTAALERILQKFHLPITYDLS-----------HPDELFHAITHDKKARGSQLNIILLEKIGQAKIV---PVPTETFKDYLGGL 1SG6 Chain:B ((8-392)) -----SILGRESIIADFGLWRNYVAKDLISDC-SSTTYVLVTDTNIGSIYTPSFEEAFRKRAAEITPSPRLLIYNRPPGEVSKSRQTKADIEDWMLSQNPPCGRDTVVIALGGGVIGDLTGFVASTYMRGVRYVQVPTTLLAMVDSSIGGKTAIDTPLGKNLIGAIWQPTKIYIDLEFLETLPVREFINGMAEVIKTAAISSEEEFTALEENAETILKAVRREVTPGEHRFEGTEEILKARILASARHKAYVVSA----GGLRNLLNWGHSIGHAIEAILTP-QILHGECVAIGMVKEAELARHLGILKGVAVSRIVKCLAAYGLPTSLKDARIRKLTAGKHCSVDQLMFNMAL--------KKIVLLSAIGTPYETRASVVANEDIRVVLA--

General information: TITO was launched using: RESULT: Template: 1SG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1748 -14055 -8.04 -42.21 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -8.04 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_1SG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG6-query.scw PDB file : Tito_Scwrl_1SG6.pdb: