Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVIVVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADATGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKEILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRSAEMVIEFVKHLV 4QEZ Chain:A ((2-225)) -RIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTTILLERYKPEKVINTGSAGGFHHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFKADEALVALAEKCMQ-----VVKGMIATGDSFMSDPNRVAAIRDKFENLYAVEMEAAAVAQVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVK------

General information: TITO was launched using: RESULT: Template: 4QEZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1294 -126785 -97.98 -581.58 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -97.98 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_4QEZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QEZ-query.scw PDB file : Tito_Scwrl_4QEZ.pdb: