Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITANTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFITAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVDTLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLD--FADVKTVMENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEAQDASDIVANAATGDVNIILGTSINEEM-GDEIRVTVIATGIDESKKERKSSRPARQAQMQSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNREETKSHGDDELNTPPFFRRKK 1W5F Chain:B ((24-326)) --------------IKVIGVGGAGNNAINRMIEIGIHGVEFVAVNTDLQVLEASNADVKIQIGENITRGLGAGGRPEIGEQAALESEEKIREVLQDTHMVFITAGFGGGTGTGASPVIAKIAKEMGILTVAIVTTPFYFEGPERLKKAIEGLKKLRKHVDTLIKISNNKLMEELPRDVKIKDAFLKADETLHQGVKGISELITKRGYIRLTSRFARIESVMKDAGAAILGIGVGKGEHRAREAAKKAMESKLIEHPVENASSIVFNITAPSNIRMEEVHEAAMIIRQNSSEDADVKFGLIFDDEVPDDEIRVIFIAT---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1W5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1575 -73822 -46.87 -246.07 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -46.87 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_1W5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W5F-query.scw PDB file : Tito_Scwrl_1W5F.pdb: