Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEAT-GSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE 1NSY Chain:A ((1-271)) -SMQEKIMRELHVKPSIDPKQEIEDRVNFLKQYVKK-TGAKGFVLGISGGQDSTLAGRLAQLAVESIREE--GGDAQFIAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNVKARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLKELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYLEGKEVSAKVSEALEKRYSMTEHKRQVPASMFDDWWK

General information: TITO was launched using: RESULT: Template: 1NSY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1294 -9523 -7.36 -35.27 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -7.36 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1NSY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NSY-query.scw PDB file : Tito_Scwrl_1NSY.pdb: