Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPAILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAI-TTSKGSHLTKTVIIAMGGGAFKPRPLELEGVEDYE--NIH-YHVSNIQQYAGKKVTILGGGDSAVDWALAFEKIAP-TTLVHRRDNFRALE-HSVQALQESSVTIKTPFAPSQLLGDGKTLDKLEITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAIGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL 5UWY Chain:A ((2-306)) -NAMYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGA-PGGQMNNTFDI---ENYPGYDHISGPELAMKMYEPLEKFNVENIYG-IVQKIENFG-DYKCVLTEDASYEAKTVIIATGAK---YRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLTQFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVKTGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDSEGWIITDDHMRTSIPGIFAIGDVRQK-D-LRQITTAVGDGAIAGQGVYHYLESF-----------

General information: TITO was launched using: RESULT: Template: 5UWY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1757 -12103 -6.89 -40.48 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -6.89 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_5UWY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UWY-query.scw PDB file : Tito_Scwrl_5UWY.pdb: