Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKIEDLVMSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKVSLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLLTCERVALNFLQHLSGIASMTAAYVEALGDDCIK--VFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMSLEQIEQAITLIAGR---SRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV 5AYY Chain:C ((10-284)) ----------------LPPVTLAALVDSWLREDCPGLNYA--ALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFLP----EGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLK-------

General information: TITO was launched using: RESULT: Template: 5AYY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1398 -70860 -50.69 -262.44 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -50.69 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_5AYY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AYY-query.scw PDB file : Tito_Scwrl_5AYY.pdb: