Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTI--DADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLV--LPDTECWFDDLDQ--KQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFK-QELYLIDFEKTRLISSPDPITDATPRYMAPELFHGAN-KTVQSDLYALGIVLYEWLTQTRLQANSYHEWAVLHCQKLNVELPSS-FQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 5K00 Chain:A ((6-258)) -------------------LLKYYELHETIGTG--AK-VKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRH--QHICQLYHVLET--------ANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKP---------SLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRIS-MKNLLN-------

General information: TITO was launched using: RESULT: Template: 5K00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1065 -20205 -18.97 -88.23 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -18.97 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_5K00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K00-query.scw PDB file : Tito_Scwrl_5K00.pdb: