Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFYKNILTLSILAAISIPVFAAENENVEKLETIRIKAHPLEQTSKDFAVADTVVDQKHLTE-GAATIGDALNSEVGIYANQFGA-GSSRPVIRGQDGPRVKVLQNSSENVDVSTLSPDHAVTVDPVLAKQVEVIRGPSTLLFGAGTVGGLVNVIDNKIPTQIPENGY--EGQVGLRYNTGSDEKLASAGVTVGLGSQVALRIEGLTRDANNYIAPNYIHEGEKERRVDNTFAQGDSVNVGLSWVYD-RGYTGISYSNRRDQYGLPGHSHEYETCHIHDLSLHCGDHDHEGHSDEEAHDHEHEHGGPWIDLKSERYDFKTELNDPF--AGFQKLRAQASYTDYQHDEIEEGTIATRFQNKGYDGRIELVH--NPIADWEGVIGTQLGQQKLNLTGEE-AFMAPTTTKKWSVFALEHKQWK--DVHFELSARADQQEIDVDDNSKQDFDGSAFSYAGAANWEFAPNYKLSFVASHQERLPLAQELYANGGHFA----TNTYELGNDQLSKEKSNNVELGLHFDN-------DKLDYHLHVYHNWFDDYIYAQTLDRYKDFRLVQYTQDKARFYGAEGEIGYQITPMYKISAFGDYVRGKID-AEGNAPRIPAGRLGTKVDADFG-DGFSGSAEYYHVFNQDKIAAYETETEGYNMLNLGVAYSGQYGAKTDYRVYLKANNLLDDTVYQHASFLSNIPQVGRNFTVGVDFSF 3FHH Chain:A ((2-640)) -------------------------------ETMTVTATGNARSSFEAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDINMRGYDHRGVLVLVDGIRQGTDTG-HLN-GTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDL-LQEGQSSGFRVFGTGGTGDHSLGLGASAFGR-TENLDGIVAWSSRDRGDLRQSNGE-------T-APNDESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEA-SE-------------------S-------SNPMVDRSTIQRDAQLSYKLAPQGNDWLNADAKIYWSEVRINAQ----EYREQITKGARLENRSTLFADSFASHLLTYGGEYYRQEQGSH---TTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRYDSYRGSSDGY--KDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFSIGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYISTTVDFA--AATTMSYNVPNAKIWGWDVMTKYTT-DLFSLDVAYNRTRGKDTDTGEYISSINPDTVTSTLNIPIAHSGFSVGWVGTFADRSTHISS---KQPGYGVNDFYVSYQGQQA-LKGMTTTLVLGNAFDKEYWSPQ----GIPQDGRNGKIFVSYQW

General information: TITO was launched using: RESULT: Template: 3FHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3689 154445 41.87 259.57 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 41.87 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_3FHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHH-query.scw PDB file : Tito_Scwrl_3FHH.pdb: