Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEKFSVEKVLSVHRWTPTLFSFTMTRPSHFKFTAGQFARIGLKVGDELIVRAYSVVSSPFDETLEFFSIVVPDGAFTSNLQHLKVGDELYLE-KIPYGYLTLARYQQPLPHDLWLLATGTGLAPFLSMLQDFETWSNYQKINLVYSVRTAAELAYVDRIQEIAETFGE----GHLGFKFIPIITRDPSAPLHERLPVLIENGELEKVA-GLELSPASSHVMLCGNPQMVDDTKEALKRCGLTMN--RRGEGNIAVENYW 2QDX Chain:A ((2-254)) ----NLYTERVLSVHHWNDTLFSFKTTRNPGLRFKTGQFVMIGLEVDGRPLMRAYSIASPNYEEHLEFFSIKVPDGPLTSRLQHLKEGDELMVSRKP-TGTLVHD--DLLPGKHLYLLSTGTGMAPFLSVIQDPETYERYEKVILVHGVRWVSELAYADFITKVLPEHEYFGDQVKEKLIYYPLVTREP-FRNQGRQTDLMRSGKLFEDIGLPPMNPQDDRAMICGSPSMLEETSAVLDSFGLKISPRMGEPGDYLIERAF

General information: TITO was launched using: RESULT: Template: 2QDX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1301 -72239 -55.53 -294.85 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -55.53 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2QDX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QDX-query.scw PDB file : Tito_Scwrl_2QDX.pdb: