Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTITPKPDSVVYRVRVAVPVHLYDTFDYTLTKAQYERAQVGSRVAISFGRQNLIGIITEKVDPQESFTGNFQLKAISELLDDEPILDEQVLNLLTWSAQYYQFPIGEVMQTALPALLRQGKPMDVLFHLWKIIPCDNAEALLKRSIKQQDAYQILKLHPAGTTENILNLSGVETATLKALQKKGLVDCTLEPHDFSPAPVQLAQMPLTLNEDQKKATQHVLNAQHQYQAFLLDGLTGSGKTEVYLHIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFNCDIALLHSGLNDSKRLQAWQQA--QTGKASIILGTRSAIYT------------PLPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGHLQGCPVLLGSATPSIDSYHLVET--GKLTALQLNQRAGHALLPKMHLIDLKIVKKQHGISQPLIEQIKNTLARKEQVLIFLNRRGYAPVLVCESCGWQANCPHCDAHFTLHTQPYSYLHCHHCGTVHRLPDHCPECQQKSLKTLGAGTAKVEEHLQELFPDHDVIRVDRDSTSRVGSWQKIYDRIQQNKPSILLGTQMLAKGHHFPHVTLVAILDIDAGLLSVDIRAPERTAQLIVQVAGRAGRGEHKGHVYLQTLRPDHPLLTTLIEKDYRAVAKQTLAERKVALLPPYRYAVLIRAESKDRDYTLHFLNEAAEQLRQIAGGIVDIWGPIPAPMERKAGRYRAHMVILSADRTRLHFYLRQWWAQLVHAPRQHQLRLSIDVDPQEFS 4U7D Chain:C ((43-375)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EKFRPLQLETINVTM---AGKEVFLVMPTGGGKSLCYQLPALC---SDGFTLVICPLISLMEDQLMVLKQL-GISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWG-HDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFTFTASF---NRPNLYYEVRQKPSNTEDFIEDIVKLINGRY-KGQSGIIYCFSQKD-------------------------------------------------------------SEQVTVSLQNL--GIHAGAYHANLEPE--DKTTVHRKWSANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSM------------ENYYQESGRAGRDDMKADCILYYGFGD---------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4U7D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1527 -26452 -17.32 -83.44 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.57 3D Compatibility (PKB) : -17.32 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_4U7D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U7D-query.scw PDB file : Tito_Scwrl_4U7D.pdb: