Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKAVLPVAGLGTRFLPASKSIPKEMVTVVDRPAIEYVVREAVEAGIEQIILVTHSSKASIENYFDRNLELETTLEQKKKFDLLAEITQIVPEHVSVVSVRQPQPLGLGHAVLCAKSVVGQDDFAVLLPDVLVKDS---SGQNDLSRMISRYNSSQAAQIMVEAVPDHLVDQYGIVDVKHSP-NEGESIAMQGIVEKPAVGSAPSNLSVVGRYVLPAKIMQLLENTPKGAGNEIQLTDAIAMLQDTDTVEAYRMQGQTFDCGSKIGYLKAVLHYGIEHPKLGNDFKKLIQELKL 2E3D Chain:B ((7-296)) KVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQYVVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEK---RQLLDEVQSICPPHVTIMQVRQG--KGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFDETGHSQIMVE--PVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRYVLSADIWPLLAKT--------QLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAFVEYGIRHNTLGTEFKAWLEE---

General information: TITO was launched using: RESULT: Template: 2E3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1451 -85952 -59.24 -314.84 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -59.24 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_2E3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E3D-query.scw PDB file : Tito_Scwrl_2E3D.pdb: