Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGIKTIGVVGVGVIGASWTALFLYKGFKVKVYGPYPIDEELFKKRIQANLSDLLALDQQTDSSHHLQDIFLNLELYNNLKDAVIDADFIQENAPERLDLKQNLYQEITSYCPEKTLIASSSSGLKVSDFQKDATHPERIFLGHPFNPPHLLPLVEIVGGKLTDPQILKKASEFYQSLGKHPIVLNKEVKGHVANRLQAALWREAFSLVKEGVCSAEDVDIAITSGPGLRWALFGPYINMELANQKGFKEAIHHLGPPMTEWWNDMQNFQHSEETTELLEEQTKELLTHYKDIDLSQKRDKGLVDILKLKQQLELD 1ZEJ Chain:A ((12-238)) ---HMKVFVIGAGLMGRGIAIAIA-SKHEVVLQDVSEKALEAAREQIPEE-------------------LLSKIEFTTTLE-KVKDCDIVMEAVFEDLNTKVEVLREVERL-T-NAPLCSNTSVISVDDIAERLDSPSRFLGVHWMNPPHVMPLVEIVISRFTDSKTVAFVEGFLRELGKEVVVC-KG--QSLVNRFNAAVLSEASRMIEEGV-RAEDVDRVWKHHLGLLYTLFGPLGNLDYIGLDVAYYASLYLYK-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1007 -119819 -118.99 -527.84 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -118.99 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_1ZEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZEJ-query.scw PDB file : Tito_Scwrl_1ZEJ.pdb: