Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCYSFSNSKAKGDVMKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSKITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLDLQLVHIKKEDP-LFSF-FGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSRE-EFGKESLYRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQ-IGIKRIRKSSTQIGEMIEYSEAVYHTELHPYHIDYET 2P19 Chain:B ((7-146)) ------------------------------------------------------------------------------------------------------------DPKTRVLEHRLLAASSAIAEKLGVSAGDEVLLIRRLRSTGDIPVAILENYLPPAFND-VSLDELEKGGLYDALRSRGVVLKIANQKIGARRAVGEESTLLDIEDGGPLLTVERVALDNSGQVIELGSHCYRPDMYNFETTL--

General information: TITO was launched using: RESULT: Template: 2P19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 639 7569 11.85 55.65 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 11.85 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_2P19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P19-query.scw PDB file : Tito_Scwrl_2P19.pdb: