Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHTTQLYQHVPETRWPIVYSPRYNITFMGLEKLHPFDAGKWGKVINFLKEEKLLSDSMLVEAR-----EASEEDLLVVHTRRYLNELKWSFAVATIT---------EIPPVIFLPNFLVQRKVLRPLRTQTGGTIMAGKLAVER--GWAINVGGGFHHCSSDRGGGFCAYADITLAIKFLFERV-EGISRATIIDLDAHQGNGHERDFMDDKRVYIMDVYNR--HIYPGDRFAK---------QAIRRKVELEWGTEDDEYLDKVERNIKKSLQEHLPDVVVYNAGTDILEGDRLGGLSIS-----PAGIVKRDELVFRMVRGRRVPILMVTSGGYQKR-TARIIADSILNLFGLGLIGPESPSVSAQNSDTPLLPPAVP 4BZ7 Chain:A ((2-368)) --------------SVGIVYGDQYRQLC----CSSPKFGDRYALVMDLINAYKLIPELSRVPPLQWDSPSRMYEAVTAFHSTEYVDALKKLQMLHCEEKELTADDELLMDSFSLNYDCPGFPSVFDYSLAAVQGSLAAASALICRHCEVVINWGGGWHHAKRSEASGFCYLNDIVLAIHRLVSSTP--QTRVLYVDLDLHHGDGVEEAFWYSPRVVTFSVHHASPGFFPGTGTWNIFLNGAGRGRFSAFNLPLEEGINDLDWSNAIGPILDSLNIVIQPSYVVVQCGADCLATDPHRIFRLTNFYPSLSGYLYAIKKIL----SWKVPTLILGGGGYNFPDTARLWTRVTALTIEE-------------------------

General information: TITO was launched using: RESULT: Template: 4BZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1595 -72528 -45.47 -243.38 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -45.47 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_4BZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BZ7-query.scw PDB file : Tito_Scwrl_4BZ7.pdb: