Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------MKQFQLRRRKQMELVLPNNYVVIDEEE-----MMYLDGG--AYLSKRACQGICVALAMSPGIFIALAGAAVLTKKL-INYIKVGGLGGWLIGAAAGVLATAAGKI--AYCIGYGALNRGCDISGNPYPWDGFISATVR------------------------------------------------------------------------------------ 4Q3K Chain:A ((20-260)) QGMNISKITINQHVGVTLTCYVQDVSQEMSNMSKRPAMLIFPGGGYQFCSDREAEPIALSYLAKGYNAFVLRYSVKEHAVFPRPLIDAEDALSYLKDNAHALHINPDKIAVIGFSAGGHLATTLATEGKVRPNAVVLGYPALIR------HEKYWN-FPTPKVDQQTPEMFVFHTFEDDLVPLSHPLYIVEELSKANIPVEFHLFKSGVHGLSLGNKIVSNGLDKMIEDDVQVWFDLSCRWLDKVLDL

General information: TITO was launched using: RESULT: Template: 4Q3K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 584 -15208 -26.04 -125.69 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -26.04 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_4Q3K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q3K-query.scw PDB file : Tito_Scwrl_4Q3K.pdb: