Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVIGLGHVGLAYALLFAS-KYKVVAYDIDSVKINNLKKGILPSKNEELMKFFCEN--NLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVSKILRV-K---PNGKIILKSTVPIGFSNKLKRLFD-------TKNIIFVPEFLREGCSIYDNLYPSRIVVGD-ETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEF--IEINAPVIEAIDISNTNRKQFIVKQILER----KPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEPNLNVSI---D--NVIFEKNFELFTKQSDLIVANRWDRGLEAYK--D--KVYTRGIWIRD 3VTF Chain:A ((22-431)) ASLSVLGLGYVGVVHAVGFALLGHRVVGYDVNPSIVERLRAGRPHIYEPGLEEALGRALSSGRLSFAESAEEAVAATDATFIAVGTPPAPD-GSADLRYVEAAARAVGRGIRAKGRWHLVVVKSTVPPGTTEGLVARAVAEEAGGVKFSVASNPEFLREGSALEDFFKPDRIVIGAGDERAASFLLDVYKAVD-------APKLVMKPREAELVKYASNVFLALKISFANEVGLLAKRLGVDTYRVFEAVGLDKRIGRHYFGAGLGFGGSCFPKDTLAFIRFGESLGLEMAISKAVLRVNEYMPRYAVQLLEERLGGLRGRHVGVLGLAFKPNTDDVRESRGVEVARLLLERGARVYVHDPMAMEKARAVLGDSVTYVEDPQALLDQVEGVIIATAWPQYEGLDYRGKVVVDGRYVKK--

General information: TITO was launched using: RESULT: Template: 3VTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2060 -53583 -26.01 -141.01 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -26.01 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_3VTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VTF-query.scw PDB file : Tito_Scwrl_3VTF.pdb: