Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPAILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTSKGSHLTKTVIIAMGGGAFKPRPLELEGVEDYE--NIH-YHVSNIQQYAGKKVTILGGGDSAVDWALAFEKIAP-TTLVHRRDNFRALE-HSVQALQESSVTIKTPFAPSQLLGDGKTLDKLEITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAIGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL 5MIT Chain:A ((24-325)) ---KYDVVIIGSGPAGMTAAMYTARSEMKTLLLERG-VPGGQMNNTAEI---ENYPGYETIMGPELSMKMAEPLEGLGVENAYG-FVTGIEDHGDYKKIITEDDEFITKSIIIATGAN---HRKLEIPGEEEYGARGVSYCAVCDGAFFRNQEILVIGGGDSAVEEALYLTRFGQSVTIMHRRDKLRAQEIIQQRAFKEEKINFIWDSVPMEIKGDDKKIQSVVYKNVKTGEVTEKAFGGIFIYVGLDPVAEFVSDLGITDEAGWIITDDHMRTNIPGIFAVGDVRQK-D-FRQITTAVGDGAQAAQEAYKFVVE------------

General information: TITO was launched using: RESULT: Template: 5MIT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1801 -55533 -30.83 -186.98 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -30.83 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_5MIT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MIT-query.scw PDB file : Tito_Scwrl_5MIT.pdb: