Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANRKIVVALGGNAILSSDPSAKAQQ--EALVETAKHLVKLIKNGDDLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDEGIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAK--AEAEKSGATFKEDAGRGWRKVVASPKP-------VDIKEIETIRTLLNNGQVVVAAGGG-GIPVVKENNGHLTGVEAVIDKDFASQRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPKVEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG 4JZ8 Chain:D ((3-316)) SAGKTVVIALGGNAMLQAKEKGDYDTQRKNVEIAASEIYKIHKAGYKVVLTSGNGPQVGAIKLQNQAAA-GVSPEMPLHVCGAMSQGFIGYMMSQAMDNVFCANNEPANCVTCVTQTLVDPKDQAFTNPTKPVGRFYT-EQEAKDLMAANPGKILRED--AGR-----GWRVVVPSPRPLEIVEYGVIKTLID-NNVLVICTNGGGIPCKRE-NKVISGVDAVIDKDLATSLLAKTLNSDYLMILTDVLNACI-N----ERKLEEIKLSEILALEKDGHFAAGSMGPKVRAAIEFTQ-ATGKMSIITSLSTAVDALNGKCGTRIIK-

General information: TITO was launched using: RESULT: Template: 4JZ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1636 28302 17.30 95.29 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : 17.30 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_4JZ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JZ8-query.scw PDB file : Tito_Scwrl_4JZ8.pdb: