Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKMNKQPQNSALVVVDVQNGFT-PGGNLAVADADTIIPTINQLAGCFEN----VVLTQDWHPDNHISFAANHPGKQPFETIELDYGSQVLWPKHCIQGTHDAEFHPDLNIPTA-QLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCVAWTALDAVKQGFKTLVIEDACKGIDLNGSLEQAWQTMQQQGVIRIQSTDLLNEC--------------------------------- 1YAC Chain:A ((2-205)) TKPYVRLDKNDAAVLLVDHQAGLLS---LVRDIEPDKFKNNVLALGDLAKYFNLPTILTTSAET------------------------------------GPNGPLVPELKAQFPDAPYIARP-----GNINAWDN------EDFVKAVKATGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFN-EITRHSAWDRMSQAGAQLMTWFGVACELHRDWRNDIAGLATLFSNHIPDYRNLMTSYDTLT

General information: TITO was launched using: RESULT: Template: 1YAC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 855 -30586 -35.77 -187.64 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -35.77 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_1YAC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YAC-query.scw PDB file : Tito_Scwrl_1YAC.pdb: