Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIHLFFRNNKKSKMHLKLNNYLISGEYMNIPRFGLGTFRLKEQAVIDSVKN-ALDVGYRAIDTAQIYENEAAIGQAIAESGVSRQDLFLTTKIWVDNFAQDKFIPSLKESLQKLRTDHVDLTLIHWPAPDLGVSIPEIMQLLLEAKQQGLTKQIGISNFNIALTQQAIDTIGVEHIATNQIELSPYLQNHKLVNFLQEKNIDVTSYMTLAYGKVLQDPVLAEIAAANKATTAQIALAWALQRGFAVIPSSTKRENLISNLKAQDIELTAAEMQMIAELDRNSREVSPEPWAPVWD 1VBJ Chain:B ((12-274)) ----------------LKLSNGVM------MPVLGFGMWKLQDGNEAETATMWAIKSGYRHIDTAAIYKNEESAGRAIASCGVPREELFVTTKLWNSDQGYESTLSAFEKSIKKLGLEYVDLYLIHWPGKDKFIDTWKAFEKLYADKK---VRAIGVSNFHEHHIEELLKHCKVAPM-VNQIELHPLLNQKALCEYCKSKNIAVTAWSPLGQGHLVEDARLKAIGGKYGKTAAQVMLRWEIQAGVITIPKSGNEARIKENGNIFDFELTAEDIQVIDGMNAGHR-YGPDP------

General information: TITO was launched using: RESULT: Template: 1VBJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1474 -26673 -18.10 -101.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -18.10 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_1VBJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VBJ-query.scw PDB file : Tito_Scwrl_1VBJ.pdb: