Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIILLGPPGAGKGTQAQLICKRYNIPQISTGDMLRAAIREGTELGLKAKSVMESGGLVSDELIIGLVKERIAQPDCVNGCIFDGFPRTIPQAEALEK----EGISIDHVIEIDVPDEEIVKRLSGRRQHPASGRVYHVVYNPPKVEGKDDETGEDLVQRPDDQEETIRKRLASYHTETEQLVGFYQGRAASGENAPTYDKLDGLRTIEDVQKDLFNILDK 4QBI Chain:B ((1-187)) MNLVLMGLPGAGKGTQAEKIVEAYGIPHISTGDMFRAAMKEGTPLGLQAKQYMDRGDLVPDEVTIGIVRERLGKDDCERGFLLDGFPRTVAQAEALEEILEDIGRKIDYVIHIDVRQDVLMERLTGRRICRNCGATYHLIFHPPAKPGVCDKCGGELYQRADDNEETVAKRLEVNMKQMKPLLDFYE----------------------------------

General information: TITO was launched using: RESULT: Template: 4QBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 739 -45395 -61.43 -248.06 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -61.43 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.621

(partial model without unconserved sides chains): PDB file : Tito_4QBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QBI-query.scw PDB file : Tito_Scwrl_4QBI.pdb: