Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTINYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEEAIRNMNDGDVVVFENTRF--EDIDGKK-ESKNDTELGKYWASLGDVFVNDAFGTAHRAHASNVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLI--PKADKILIGGGMTYTFYEAKGIKIGNSLVE-------ADKVELAKELIEKAGDKLVLPIDNVCAPEFSNDVETQVIEGDIPD---GLMA----LDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTIGVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAAINDK 2CUN Chain:A ((4-399)) -----LEDFNFHNKTVFLRVDLNSPMKDGKIISDARFKAVLPTIRYLIESGAKVVIGTHQGKPYSEDYTTTEEHARV---LSELLDQHVEYIEDIFGRYAREKIKELKSGEVAILENLRFSAEEVKNKPIEECEKTFLVKKLSKVIDYVVNDAFATAHRSQPSLVGFARIK-PMIMGFLMEKEIEALMRAYYSKDSPKIYVLGGAKVEDSLKVVENVLRRERADLVLTGGLVANVFTLAKGFDLGRKNVEFMKKKGLLDYVKHAEEILDEFYPYIRTPVD------FAVDYKGERVEIDLLSENRGLLHQYQIMDIGKRTAEKYREILMKARIIVANGPMGVFEREEFAIGTVEVFKAIAD-SPAFSVLGGGHSIASIQKYGITG-ITHISTGGGAMLSFFAGEELPVLRALQ--

General information: TITO was launched using: RESULT: Template: 2CUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2233 -84996 -38.06 -225.45 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -38.06 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.649

(partial model without unconserved sides chains): PDB file : Tito_2CUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CUN-query.scw PDB file : Tito_Scwrl_2CUN.pdb: