Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLRRIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLVTLCAALLHDVGHGPYSHTFEHIFHT--NHEAITVEIITSPETEVYQILNRVEEGFPEKVASVIQ---KTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGIAFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELHSQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATFSGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDGTLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGALIEIN 2HEK Chain:A ((1-369)) -----------MIKEFSDPLYGFVRVG-EAGLRLIDSFPFQRLRYVKQLGLAYLVFPSAQHTRFEHSLGVYHITERICESL--------------KVKEKELVKLAGLLHDLGHPPFSHTTEVLLPRERSHEDFTERVIKE-TEIYEILKQDYSHEDIERLVRITLGKPEDEEEKLLSEIITGEFGSDRMDYLRRDAYFCGVSYGFFDYDRLISTLRVYENKVVVDESGLRALENFLISRYFMYVQVYFHKVVRILSIHLVEFLKKLISQED-------------------FTDINNFLRLNDAFVISELFKRKAF-----REDFERIFQRKHFKTLLSTEN-YEKFSETKERLLEKFPQEKVRFDEVEKEV-------------YGGNIYVLSSEG-LKKAHELSPLIASL--KPIKLYRIYVDRQ---------------LWEKARSELK--------

General information: TITO was launched using: RESULT: Template: 2HEK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1788 -5830 -3.26 -16.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -3.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_2HEK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HEK-query.scw PDB file : Tito_Scwrl_2HEK.pdb: