Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------FDCTIER--RLGTQTKARARTSAKAMPEMIPAESGKHTVIAGTRVWVYGDCRTYP-THLPDGSTISGRPFQASDVEEDCIELDVHGKPKQ----------------- 1HM9 Chain:A ((261-468)) VSFVNPEATYIDIDVEIAPEVQIEANVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPNSSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTIT-----------VNYDGKNKYKTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAI---AIGRGRQINKDEYATRLPHHPKNQ

General information: TITO was launched using: RESULT: Template: 1HM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 392 532 1.36 7.28 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 1.36 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_1HM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HM9-query.scw PDB file : Tito_Scwrl_1HM9.pdb: