Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKDGIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQ-GMIPVTSDTKEITDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYTGPRTILHNLRKTVERAGVQVENVIISPLAMVQSVL-NEGEREFGATVIDMGAGQTTVATIRN-QELQFTHILQE-GGDYVTKDISKVLKT-SRKLAEGLKLNYGEAYPPLASKETFQVEVIGEVEAVEVTEAYLSEIISARIKHILEQIKQEL----DRRRLLDLPGGIVLIGGNAILPGMVELAQEVFGVRVKLYVPNQVGIRNPAFAH-VISLSEFAGQLTEVNLLAQ-GAIKGENDLSHQPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQ-NKPKLADRFRGLIGSMFDE 4I6M Chain:B ((3-439)) PFRQDSI--LIIYPRSQTTLVQFGLNEE--------------------TFTVPELEIPTQIYRTTRQDG-SYTYHSTNKDNKAELIKPIQNGEIIDISAFTQFLRLIFVSILSDRANKNQDAFEAELSNIPLLLITHHSWSQSDLEIITQYVFE-----------------------SLEINNLIQLPASLAATYSMISLQN---CCIIDVGTHHTDIIPIVDYAQLDHLVSSIPMGGQSINDSLKKLLPQWDDDQIESLKKSPIFEVLSKNSDLEFNTFWDEKGNEIKVGKQRFQGC-NNLIKNISNRVGLTLDNIDDINKAKAVWENIIIVGGTTSISGFKEALLGQLLKDHLIIEPEEEKSKREEEAKSFVPTIEYVQCPTVIKLAKYPDYFPEWKKSGYSEIIFLGAQIVSKQIFTH---------------PKDTFYITREKYNMKGPAALWDVQF--------

General information: TITO was launched using: RESULT: Template: 4I6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1870 -19333 -10.34 -50.35 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -10.34 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4I6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I6M-query.scw PDB file : Tito_Scwrl_4I6M.pdb: