Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFQLKTLSVFLSALGAFSYSSVAQAQLMFSQYVDGSSNKKGLEIYNPDGTTVNLADYEIQQFNNGGTAKTATFRLQGTLASEQKFLVGRSELQTELGNKVNQVAALSFNGDDAVVLVYKGTPVDRFGRIGERPETGWGTAVSSLGNSFKRIETENPALSIDPTAAFDLDHSWTAWANRNDFTNLSGSTTQPPIETVSCSSSDTPIADLATSAQNQTYTVRGVITADYRYANGFSGFYVQTPDTKARANVSNAIFVYIPNSSTVKGGQIGDEVILRGRLTSYQNQLQIDQLQQDIQTCNSNMANQVQPISLELPFSSLTGGSTHSPQRYQGMLVKLPQTLMVSENYNYGRYGELSLSLGRLYIPTNLYPALSPEAKALAQKNLLSKIIFDDGYNNQNRTPWLPTNFSAANTLRSGYQLKNVEGILEYRFNGWRVQPVLGRTQPEVIAQTNSRQNVIAKNANHIRVASFNVLNYDNGATGFPTERGANTQAEFDKQHRKIVSALKSIDADVYGLMEIANNGYGPNSAIAHLTSALGPDWKYVIPENLDRLGTDVIAVAIIYNSKRVKPLNKAVVLD-LGEKNRTTLAQTFQAIRGNKTFTVIPNHLKSKGCSGVDANSSDADQNDGQGCWNPTRVKAVDQIVQWLAKNPTQVPKQNALLVGDMNSYAKEAPILAFEKANYKVLLNDTKVGQ--GAQAYSYVFGVASDA---NGNGGAGNLDHAIADADLYPKVVRTFAWHINADEPTVLDYNEEYKTDEQKALFYGEDAYRSSDHDPVIVDLDLNGKDSNQPNDNQKSPIFDFLSQLMEWISQLFKRS 4F1H Chain:B ((2-217)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DSKLSIISWNVDGLDTL--------------NLADRARGLCSYLALYTPDVVFLQELIPAY------VQYLKKRA-VSYLFFEGS------DDGYFTGIMLRKSRVKFLESEIICFPTTQMMRNLLIAQVTFS--GQKLYLMTSHLESCKNQ------------------SQERTKQLRVVLQKIKEAP---EDAIVIFAGDTNLRDAEVANVGGLPAG-V--CDVWEQLGKQEHCRYTWDTKANSNKTVPYVS--RCRFDRIFLRSAKTA---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4F1H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1007 -72694 -72.19 -346.16 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -72.19 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_4F1H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F1H-query.scw PDB file : Tito_Scwrl_4F1H.pdb: