Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MLNLKSSHST------AFTPSSPAERYAEALASG-QFMADEAQAQAVQELDRVWKELLNRYKASKKAFRRFRRQTSPKGVY---MWGSVGRGKTWLMDQFYESVPFRRKTRMHFHHFMQHVHKELNKLSGQRNPLDIVADQIYKDAVVICFDEFFVSNVTDAMILSDLFQKLFVRGVTLIATSNIAPDGLYKN----GIHRDRFIPTIEMVKKNCVVLNVDAGVDYRLRVLKQAQLFKSPLGNEAQSWISECYTALTHTQTNSHEPIVINNRVVETLGHTEDVLWCEFSELCLKPRSPAD----FIEIANIYNTVLVSNVPHLTDFLSEGTRRFIYLVDEFYDRGVKLLLTSQDSIIDIYQGEKLAFEIERTRSRLLEMQSDEYLHSEHRHIDATKTS 5I3E Chain:A ((9-356)) MAISPRDEQNRSVDLWFAYKVPKLTKDADSDSASGYEYVYYDRQVGAVQKSPNLMNDPKGALFYTLDSVFGDPGDTTGWILYNDEMPADANRSNNATLGHTKGVIAFDIASS----SALWLLHSWPKYASPSVVPTPLYG----QTFLCLSLDLATAGKLAAQMALHQQPQVYLPRTGGLDHTSPLYALTQPLNASAPGDSDSLDFKTRGGVPFKVIAKNRKWGKDFWNDLVGPT--LKADMYVE--TWIR-----------GKIPPVLDSDGVHKTY----DIKFIDLRKLGAPWAWPETQDHAKWGITTTDNWVCVGDINRMVTQEKRG--------------GGTIAFQDPKLWKALCETDLIIPPPGKT-----DAQARAMIRKTHEP-------

General information: TITO was launched using: RESULT: Template: 5I3E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1928 70219 36.42 214.74 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 36.42 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.078

(partial model without unconserved sides chains): PDB file : Tito_5I3E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I3E-query.scw PDB file : Tito_Scwrl_5I3E.pdb: