Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MSEKLQKVLARIGLGSRRYMEEVIAAGRVSVNGRVAQVGERIEPGDELRIDGRKVQFQIEDEIRRRVLIYYKPEGEICSRNDPEKRPTVFDHLPQIANDRWVMVGRLDINSTGLLLFTNDGELANRLMHPSNEIEREYAVRVMGEVTPQLRQNMVNGVELEDGPAKFESFSEIGGEGINRWYQVVVKE--GRNREVRRIFESQG----LKVSRLLRTRYGTVILPRELRTGRWMELDKTDIDNLAKSVELKPRQGTGLFGMAKRR------TERMTEKPMAARRGGYLRQQRRDDEKEAPANTGNQRKS--TGFNRGFKKF 4F3V Chain:A ((1-275)) GHMTDRLASLFESAVSMLPMSEARSLDLFTEITNYDESACDAWIG-RIRCGDT------------DRVTLFRAWYSRRNFGQLSGSVQIS-----MSTLN----------ARIAI-----------GGLYGDITYP---VTSPLAIT-MGFAACEAAQG-----NYADAMEALEAAPVAGSEHLVAWMKAVVYGAAERWTDVIDQVKSAGKWPDKFLAGAAGVAHGVAAANLALFTEAERRLTEANDSPAGEAC---ARAIAWYLAMARRSQGNESAAVALLEWLQTTHPEPKVAAALKDPSYRLKTTTAEQIASRADPWDPGSVV-

General information: TITO was launched using: RESULT: Template: 4F3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1287 45934 35.69 180.13 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 35.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_4F3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F3V-query.scw PDB file : Tito_Scwrl_4F3V.pdb: