Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGIVS-NDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFKFHDQ-SIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRS---EETDFLNKLKSALNEVKGGFAYLLMTE-TAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFIRDVQPGEIVIINDEGIQIDC----FTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGRNLAKESPVK--ADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTP----ELREQGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCMAYFNGDYPTPLYDYEEKYQESLKEKVSFF 1AO0 Chain:A ((1-459)) -----------CGVFGIWGHEEAPQITYYGLHSLQHRGQEGAGIVATDG-EKLTAHKGQGLITEVFQNGEL-SKVKGKGAIGHVRYATG----YENVQPLLFRSQNNGSLALAHNGNLVNATQLKQQLENQGSIFQTSSDTEVLAHLIKRSGHF---TLKDQIKNSLSMLKGAYAFLIMTET-EMIVALDPNGLRPLSIGMMG-DAYVVASETCAFDVVGATYLREVEPGEMLIINDE-GMKSERFSM---NINRSICSMEYIYFSRPDSNIDGINVHSARKNLGKMLAQESA--VEADVVTGVPDSSISAAIGYAEATGIPYELGLIKNRYVGRTFI----QPSQALREQGVRMKLSAVRGVVEGKRVVMVDDSIVRGTTSRRIVTMLREAGATEVHVKISSPPIAHPCFYGIDTSTHEELIASSHSVEEIRQEIGADTLSFLSVEGLLKGIGRKYDDSNCGQCLACFTGKYPTE-IYQDTV-LPHVK------

General information: TITO was launched using: RESULT: Template: 1AO0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2797 -7387 -2.64 -16.83 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -2.64 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_1AO0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AO0-query.scw PDB file : Tito_Scwrl_1AO0.pdb: