Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRGHNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNVVYGAKNQKFGAAGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK 2NX8 Chain:A ((11-162)) --YSLEEQTYFMQEALKESEKSLQKAEIPIGCVIVKDGEIIGRGHNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHVIYGASNQKFGGVDSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRE-

General information: TITO was launched using: RESULT: Template: 2NX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 875 -115267 -131.73 -758.34 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -131.73 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_2NX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NX8-query.scw PDB file : Tito_Scwrl_2NX8.pdb: