Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIIAAMPEELAYLVQHLDNTQEQVVLG-NTYHTGTIASHEVVLVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDVTAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEAIKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRSAQVLLAFLKALD 4BMX Chain:B ((24-247)) -KIGILGAMREEITPILELFGVDFEEIPLGGNVFHKGVYHNKEIIVAYSKIGKVHSTLTTTSMILAFGVQKVLFSGVAGSLVKDLKINDLLVAIQLVQHDVDLSAFDHPLGFIPESAIFIETSESLNALAKEVANEQHIVLKEGVIASGDQFVHSKERKEFLVSEF-KASAVEMEGASVAFVCQKFGVPCCVLRSISDNADEEANMSFDAFL----EKSAQTSAKFLKSM-

General information: TITO was launched using: RESULT: Template: 4BMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1359 -50622 -37.25 -227.00 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -37.25 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_4BMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BMX-query.scw PDB file : Tito_Scwrl_4BMX.pdb: