Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALA-FIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE 5HUJ Chain:B ((37-307)) ---QEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYVLGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQ-DEADAQKALAFIAP-DQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGTDHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYE-------------LADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFADFWK

General information: TITO was launched using: RESULT: Template: 5HUJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1205 -27814 -23.08 -108.65 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.91 3D Compatibility (PKB) : -23.08 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_5HUJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HUJ-query.scw PDB file : Tito_Scwrl_5HUJ.pdb: