Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANRKIVVALGGNAILSSDPSAKAQQ--EALVETAKHLVKLIKNGDDLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDEGIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAK--AEAEKSGATFKEDAGRGWRKVVASPKP-------VDIKEIETIRTLLNNGQVVVAAGGG-GIPVVKENNGHLTGVEAVIDKDFASQRLAELVDADLFIVLTGVDYVFVNYNK-PNQEKLEHVNVAQLEEYIKQDQFAPGSMLPKVEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG 4JZ9 Chain:A ((3-316)) SAGKTVVIALGGNAMLQAKEKGDYDTQRKNVEIAASEIYKIHKAGYKVVLTSGNGPQVGAIKLQNQAAA-GVSPEMPLHVCGAMSQGFIGYMMSQAMDNVFCANNEPANCVTCVTQTLVDPKDQAFTNPTKPVGRFYT-EQEAKDLMAANPGKILRED--AGR-----GWRVVVPSPRPLEIVEYGVIKTLID-NNVLVICTNGGGIPCKRE-NKVISGVDAVIDKDLATSLLAKTLNSDYLMILTDVLNACI-NYKKPDERKLEEIKLSEILALEKDGHFAAGSMGPKVRAAIEFTQ-ATGKMSIITSLSTAVDALNGKCGTRIIK-

General information: TITO was launched using: RESULT: Template: 4JZ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1628 29451 18.09 97.84 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 18.09 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_4JZ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JZ9-query.scw PDB file : Tito_Scwrl_4JZ9.pdb: