Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMFTLKQFQYFIKIVEEGSFTAASEKLFIAQSALSRQMKLLEEEIDFLLFDRTDKRVKLTAAGEVFYKKIKDNLL-YLNEIIDLSKSVSEGKNRQIKIAHSSSIVVDNTKVQILKEVSLTQQLSFEINTLSSEQQILALLNGEIDIGLIRPPVRHTLDGINTLKL-YEEPLMVAVHIDHAKFAKSEKVYLKDLKDEC-FVSTPHAERGGLSYLVANLCLAAGFTPQKASIQSRKISQ-LQL-VAANLGVGIVPAEFQQ--ILPANVKL-IPLEDSLSLSEVLLVYRKDH--DEIIQHCAEQIHQMFLS 3FZV Chain:A ((4-300)) ---YTLRQLKYFVTTVECGSVAEASRKLYIAQS-ISTAVKGLEESFV---------QLFLTPAGARFYRKAQELLRMAHEFEQNLADN--DVIAGQIDIGCFETVAPLYLPGLIAGFRQAYPGVEIRIRDGEQQELVQGLTSGRFDLAFLYEH--DLDSTIETEPLMPPQRPHALLPEGH-RFAGQAQVSLRDLCLEPMILLDVQPSRTYFVSLFEE----LGLTPNIAFSSPSI--EMVRGMVGQGFGFSLLVTRPHSECTYDGKKVVMVDLAEPVSTSGLAAAWLKRAQLTKPARLFVDYCREQLGK

General information: TITO was launched using: RESULT: Template: 3FZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1252 -18385 -14.68 -66.85 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -14.68 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_3FZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZV-query.scw PDB file : Tito_Scwrl_3FZV.pdb: