Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MVRINKMNGKHVDIYI-GKLNDFLNRKQHDFPDFRTFNEYKKQQIQLIKNKLIAQKLEIAPTDLVFAKHEYGKPYLLDHALH--FNHSHSQQYYALALSERV-KDIGIDVEELDRKVRLDSLAQHAFHPDEYATWQSLEQDREYWFKVWTTKEAVLKASGLGIRLDLNTLNTQAPPTNHGGLCSHELIGTFAYQNFVLGNMILTVAWRSEQSCRGFQFPSIQIHSLDR 5C50 Chain:B ((5-189)) SDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALAGQLP--------AVGRSMCVEISLKTSEGDSMELEIWCLEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTP-AYRLSRKQGHEYVILYRIYFGEV-QLSGLGEGFQTVRVGTVGTPV---------------------GTITLSCAYRINLAF---------------

General information: TITO was launched using: RESULT: Template: 5C50.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 791 -1309 -1.65 -7.35 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -1.65 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_5C50.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C50-query.scw PDB file : Tito_Scwrl_5C50.pdb: