Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIEDVVKLGPVIPVLAFDSAEQGEHVSRALHAGGVKVLEITLRTAAGLAAIERASQLADDIVVGVGTITKPEHCAQAKKAGAKFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQAIELGYDIVKFFPAQQAGGVEMLKAFYGPFPNLRFCPTGGITAETAPDFLKQPNVVCVGGSWLTPKPVVAAQDWAEITRLAQIASQL 4BK9 Chain:C ((11-209)) --DIDSVMRLAPVMPVLVIEDIADAKPIAEALVAGGLNVLEVTLRTPCALEAIKIMKE-VPGAVVGAGTVLNAKMLDQAQEAGCEFFVSPGLTADLGKHAVAQKAALLPGVANAADVMLGLDLGLDRFKFFPAENIGGLPALKSMASVFRQVRFCPTGGITPASAPKYLENPSILCVGGSWVVPAGKP---DVAKITALAKEASA-

General information: TITO was launched using: RESULT: Template: 4BK9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1107 -176748 -159.66 -888.18 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -159.66 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_4BK9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BK9-query.scw PDB file : Tito_Scwrl_4BK9.pdb: