Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MNHVMCVMSYSLKMVLNHEEDTQRLAQALAQHVQAG-----VIYLIGDLGAGKTTLTRYFLQALGHKGSVKSPTYTLVEPYKINNKEIFHFDLYRLNDPYELELMGIRDYLDIQDALFLFEWPSKGGDEIPEADIVIDIQKSDDELNRFVTLTL-PTEHLYQTLQEQLHD----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1FNN Chain:A ((2-389)) AIVVDDSVFSPSYVPKRLPHREQQLQQLDILLGNWLRNPGHHYPRATLLGRPGTGKTVTLRKLWELYKDKTTA----------------RFVYINGFIYRNFTAI-IGEIARSLNI----------PFPRRGLSRDEFLALLVEHLRERDLYMFLVLDDAFNLAPDILSTFIRLGQEADKLGAFRIALVIVGHNDAVLNNLDPSTRGIMGKYVIRFSPYTKDQIFDILLDRAKAGLAEGSYSEDILQMIADITGAQTPLDTNRGDARLAIDILYRSAYAAQQNGRKHIAPEDVRKSSKEVLFGISEEVLIGLPLHEKLFLLAIVRSLKISHTPYITFGDAEESYKIVCEEYGERPRVHSQLWSYLNDLREKGIVETRQNTTLISIGTEPLDTLEAVITKLIKEELR

General information: TITO was launched using: RESULT: Template: 1FNN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 414 1788 4.32 13.05 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 4.32 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_1FNN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNN-query.scw PDB file : Tito_Scwrl_1FNN.pdb: