Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILIVHAHPEPLSFTTSLKTTAQQTFKKLGHQVVISDLYAMQFNPLASKEDFL-ELNQPDYFNYALEQRNATKQQLLSPDIQAEIDKVKRADLIILNFPLYWTSVPAILKGWIDRVFVSGLFYGGKRFYNHGGMAGKKAMLCLTLGGRTHMFGENSIHGPIEHYLSPIQRGTLAYTGFEVLPPFIAYHVPYISQEARETILEDYVTYLTHLDQLEPLKFPKLEEFDEKLYPL 3NHF Chain:B ((4-220)) -KVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPI---------------

General information: TITO was launched using: RESULT: Template: 3NHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1027 -85345 -83.10 -395.11 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -83.10 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_3NHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NHF-query.scw PDB file : Tito_Scwrl_3NHF.pdb: