Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYANGGDEMEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFILLILKEK--DGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVS-EGRLSLVYLTEQGRQFAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK 3JW4 Chain:C ((12-147)) -----------------DTPYSYLIRSIGMKLKTSADARLAELGLNSQQGRMIGYIYENQESGIIQKDLAQFFG---ASITSMLQGLEKKGYIERRIP--RQKNIYVLPKGAALVEEFNNIFLEVEESITKGLTKDEQKQLMSILIKVNRS----

General information: TITO was launched using: RESULT: Template: 3JW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 357 14630 40.98 116.11 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : 40.98 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_3JW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JW4-query.scw PDB file : Tito_Scwrl_3JW4.pdb: