Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSCYLITGGAGFIGSTLANHLG-EEHVVIVVDDLSMGKVE---NLDM------TKNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDFVYIDDVIQALLLVANEE-AALGKQFNVGTGKATTLLELIHAINEILGVELTLE-YKEERSGDIHDSLADITKIQS-IGYQPKYGILSGMTNYLKTEIN 3RU9 Chain:B ((25-346)) PKTWLITGVAGFIGSNLLEKLLKLNQVVIGLDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVM--KGVDHVLHQAALGSVPRSIVDPITTNATNITGFLNILHAAKNAQ--VQSFTYAASSSTYGDHPALPKVEENIGNPLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIP----KWTAAML-KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGYIYDELNLIHHI-KYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRLSMPWYV-

General information: TITO was launched using: RESULT: Template: 3RU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1734 -20462 -11.80 -67.31 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -11.80 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_3RU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RU9-query.scw PDB file : Tito_Scwrl_3RU9.pdb: